Anti-Protein Phosphatase 4 (formerly X) Catalytic Subunit (PPP4C, PP4C, PP4, Protein Phosphatase X,

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions. The serine/threonine protein phosphatases (PP) are intimately involved in this process. Protein phosphatase 4C may be involved in microtubule organization. PP4 consists of a catalytic subunit, PPP4C, and a regulatory subunit, PPP4R1, and belongs to the PPP phosphatase family. More specifically it is part of the PP-X subfamily. PP4 is specifically associated with NF-kappa B, p50, c-Rel, and RelA, which are pleiotropic transcription factors that play central roles in the immune and inflammatory responses, as well as apoptosis. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.