Anti-Protein Kinase C, iota, lambda (PKCi, PKC, Atypical Protein Kinase C lambda/iota, aPKC lambda i

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups by the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising alpha, betaI (also known as beta1), betaII (also known as beta2) and gamma), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation, novel (n)PKC isoforms (comprising delta, epsilon, eta (also known as PKC-L), theta and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+, and atypical (a)PKC isoforms, namely zeta, iota, and lambda (the mouse homolog of human PKC iota) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCnu. , PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. , PKCiota/lambda (nPKC-iota, aPKC-lambda/iota) may play a role in the secretory response to nutrients and is involved in cell polarization processes and the formation of epithelial tight junctions. PKCiota/lambda is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PKCiota/lambda functions in both pro- and anti-apoptotic pathways. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.