Anti-Protein Kinase C eta (PKCeta)

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups with differing enzymes cofactor requirements. The first is conventional (c) PKC isoforms (comprising alpha, betaI (also known as beta1), betaII (also known as beta2) and gamma)), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation. The second is novel (n)PKC isoforms (comprising delta, epsilon, eta (also known as PKC-L), theta and µ (the mouse homolog of human PKCµ, known as PKD)) that require DAG but not Ca2+ for activation. The third is atypical (a)PKC isoforms, namely zeta, iota and lambda (mouse homolog of human PKC iota) that require neither Ca2+ nor DAG for activation. A new PKC member has recently been discovered and is referred to as PKCnu. , PKC family members phosphorylate a wide variety of protein targets and are known to be involved in many diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. , PKCeta (PKC eta) that is endogenously expressed or overexpressed is found to associate with cyclin E/cdk2/p21 complex in keratinocytes of mice and humans. Defects in PRKCH may be a cause of susceptibility to ischemic stroke, known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event that leads to the death of brain neural tissue causing the loss of motor, sensory and/or cognitive function. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.