Product information "Anti-PLP (Proteolipid Protein)"
Human PLP (also known as lipophilin) is a 29,946 dalton protein (277 amino acids) found in myelin of the CNS and PNS. Expressed by oligodendrocytes and Schwann cells, PLP stabilizes myelin by preventing lipid bilayer fusion, and aids in its compaction. Different mutations of the human PLP gene product result in two neurological disorders -- Pelizaeus-Merzbacher disease and Spastic Paraplegia type 2. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:500, Immunohistochemistry: 1:100-1:200, Immunocytochemistry: 1:100-1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.