Anti-Plasminogen Activator Inhibitor Type 1 (PAI 1) (HRP)

Type I plasminogen activator inhibitor, PAI 1, is a 50kD single- chain glycoprotein which belongs to the serine protease inhibitor (SERPIN) family of proteins. The concentration of PAI 1 in normal human plasma is very low, with an average concentration of ~20ng/ml. The plasma concentrations of PAI 1 can be affected by a number of factors including diurnal variations, age, sex, pregnancy, obesity and exercise status. PAI 1 is synthesized by various cell types including endothelial cells, hepatocytes, vascular smooth muscle cells, fibroblasts, mesothelial cells, granulosa cells and malignant cell lines. It is also found in the alpha-granules of platelets as well as plasma. PAI 1 exists in at least three different conformations, including an active form with a half-life of ~1-3 hours, a latent form and a proteolytically or oxidatively inactivated form. The plasma glycoprotein vitronectin has been shown to bind active PAI 1 and stabilize it in its active conformation, extending its functional half-life to greater than 24 hours. Little specific binding of the latent or inactive forms of PAI 1 to vitronectin occurs. PAI 1 is considered to be the primary regulator of plasminogen activation in vivo. It is the major physiologic inhibitor of both the single chain and two-chain forms of tPA, being able to inhibit the single-chain form at least 10000 times greater than other PAIs. Applications: Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Rz Ratio (Reinheitszahl, A403/A280): 0.41 , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.