Anti-Peroxidase, Horseradish (HRP)

Horseradish Peroxidase (HRP) is a hemoprotein catalyzing the oxidation by hydrogen peroxide of a number of substrates such as ascorbate, ferrocyanide, cytochrome C and the leuko form of many dyes. Horseradish Peroxidase has been found well suited for the preparation of enzyme conjugated antibodies, due in part to its ability to yield chromogenic products and to its relatively good stability characteristics. Applications: Suitable for use in ELISA, Dot Blot, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: ELISA: 1:10,000-1:40,000, Western Blot: 1:1000-1:4000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.