Anti-PEAR1 (Platelet Endothelial Aggregation Receptor 1, MEGF12)

PEAR1 (platelet endothelial aggregation receptor 1, also MEGF12) is a 150kD member of the MEGF family of proteins. It is expressed principally on platelets and endothelium, and undergoes phosphorylation in response to platelet activation and adhesion. Mature human PEAR1 is a type I transmembrane protein that is 1017 amino acids (aa) in length. It contains a 735 aa extracellular region (aa 21-755) and a Pro-rich 261 aa cytoplasmic domain. The extracellular region shows one EMI segment (aa 25-103) followed by nine EGF-like repeats (aa 104-692). There is one potential alternate start site at Met376. Applications: Suitable for use in Direct ELISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml. Use 10ul to label 1-5x10e5 blood derived CD41+ platelets. , Direct ELISA: 0.5-1.0ug/ml. Detection limit for rhPEAR1 is~ 0.3ng/well. , Western Blot: 0.1-0.2ug/ml. Detection limit for rhPEAR1 is ~ 1ng/lane and 10 ng/lane under non-reducing and reducing conditions, respectively. , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.