Anti-NUMB

Anti-NUMB
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N8350-03.100 100 µl - -

4 - 22 business days

496.00€
 
Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal... more
Product information "Anti-NUMB"
Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for a-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms and they are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, therefore two daughter cells have different responses to Notch signaling and have different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, Immunofluorescence: 1:400, Flow Cytometry: 1:400, Immunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: N8350-03

Properties

Application: FC, IF, IP, WB
Antibody Type: Monoclonal
Clone: 8A23(C29G11)
Host: Rabbit
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic peptide (Blue Carrier-coupled) corresponding to residues surrounding Ala570 of human Numb protein.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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