Anti-NORE1 (RASSF1)

RASSF1A, a new tumor-suppressor gene located at 3p21.3 is frequently inactivated by promoter region hypermethylation in a variety of human cancers including lung, breast, kidney and neuroblastoma. Another member of the RASSF1 gene family, Ras effector NORE1, which is structurally related to RASSF1, can also mediate a Ras-dependent apoptosis. Moreover, an analysis of NORE1 protein expression showed that it is frequently down-regulated in lung tumor cell lines and primary lung tumors. The RAS oncogene is mutated in nearly one third of all human cancers. Members of the RAS superfamily are plasma membrane GTP binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including NORE1, share a RAS association (RA) domain. NORE1 mediates a Ras dependent apoptosis. NORE1 exists in at least two spliced isoforms, A and B. Transcript A encodes a protein of 418 aa (~47kD) while transcript B contains an ORF of 265 aa (~30.5kD). NORE1A and NORE1B are expressed in most of the normal tissues analysed and are potential tumor suppressors. , Applications: , Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.