Anti-NHERF1 (Na+/H+ Exchanger Regulatory Factor, Ezrin-Radixin-Moesin Binding Protein 50)

Anti-NHERF1 (Na+/H+ Exchanger Regulatory Factor, Ezrin-Radixin-Moesin Binding Protein 50)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N2025-02B.100 100 µl - -

3 - 19 business days

505.00€
 
Na+/H+ exchanger regulatory factor (NHERF1or EBP-50) is a member of the PDZ domain-containing... more
Product information "Anti-NHERF1 (Na+/H+ Exchanger Regulatory Factor, Ezrin-Radixin-Moesin Binding Protein 50)"
Na+/H+ exchanger regulatory factor (NHERF1or EBP-50) is a member of the PDZ domain-containing proteins (1). NHERF1 was first identified as a necessary cofactor for cyclic AMP-associated inhibition of Na+/ H+ exchanger isoform 3 (NHE3) (2). NHERF1 is a multifunctional adaptor protein that interacts with receptors and ion transporters via its PDZ domains and with the ERM family of proteins including merlin via its carboxy-terminus (2,3). NHERF1 has been found to promote the formation of a ternary complex containing PTEN, NHERF1 and PDGFR. Thereby NHERF1 may function to recruit PTEN to PDGFR to inhibit the activation of the PI3K/Akt signaling in normal cells, and this mechanism may be disrupted in cancer (4). NHRF1 also binds to the cystic fibrosis transmembrane conductance regulator (CFTR), which functions as an ion channel and has disease-causing mutations in Cystic fibrosis (5). Other proposed functions of NHERF1 include testicular differentiation, endosomal recycling, membrane targeting, protein sorting and trafficking (6). , Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: N2025-02B

Properties

Application: WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human
Immunogen: Synthetic peptide (KLH-coupled) surrounding Ala140 of human NHERF1.
Purity: Purified by peptide affinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Anti-NHERF1 (Na+/H+ Exchanger Regulatory Factor, Ezrin-Radixin-Moesin Binding Protein 50)"
Write a review
or to review a product.
Viewed