Anti-Neutrophils (Allotypic Marker) (FITC)

Ly-6B. is a ~25-30kD GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B. to ~15kD (Rosas et al.2010). N2254-01G has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat-1:10. 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Y3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.