Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. , , Recommended Dilutions: , Flow Cytometry: 2.5ug per 10e6 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled monoclonal antibody may , be visualized by adding a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. , Optimal dilutions to be determined by the researcher. , , Storage and Stability:, Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.