Anti-Neuron Specific Enolase (NSE)

Applications:, Suitable for use in ELISA. May be used for immunohistochemistry on formalin-fixed and paraffin-embedded sections. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:100-1:300, Optimal dilutions to be determined by the researcher. Matched Pairs: Capture - Detection:, N2175-09A - N2175-06A, N2175-07A - N2175-06A, N2175-06A - N2175-07A, N2175-04A - N2175-06A, N2175-19 - N2175-19B, Hybridoma: Derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified neuron-specific enolase from human brain. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.