Product information "Anti-Neuron Specific Enolase (NSE)"
Human NSE-2 (EC 18.104.22.168) is a 47,138 dalton protein (434 amino acids) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-Dglycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues. Applications: Suitable for use in RWestern Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:250-1:500, Immunohistochemistry: 1:100-1:200, Immunocytochemistry: 1:100-1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.