Anti-Neurofilament NF-H

Anti-Neurofilament NF-H
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N2160-17A.100 100 µl - -

1 - 19 business days

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in... more
Product information "Anti-Neurofilament NF-H"
Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable. This antibody recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections, Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:10,000, Immunohistochemistry: 1:1000, Immunocytochemistry: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: N2160-17A


Application: ICC, IHC, WB
Antibody Type: Monoclonal
Clone: 8A326
Conjugate: No
Host: Mouse
Reactivity: Chicken, Human, Mouse, Swine, Rat
Immunogen: Native NF-H purified from porcine spinal cord
Format: Ascites

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Our products are for laboratory research use only: Not for administration to humans!
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