Anti-Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)

Anti-Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N2060-04H.100 100 µl - -

3 - 19 business days*

546.00€
 
Nestin is a Class VI intermediate filament expressed in the developing central nervous system... more
Product information "Anti-Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)"
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Westerm Blot: 1:1000-1:5000. Doublet band seen ~220-240kD., Flow Cytometry: 1ug/1x10e6 cells, Immnohistochemistry(FFPE): 1:100-1:200, Immunofluorescence (IC):: 1:50-1:200, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: N2060-04H

Properties

Application: ICC, IF, IHC, WB
Antibody Type: Monoclonal
Clone: 9A19
Conjugate: No
Host: Mouse
Reactivity: Human
Immunogen: A fragment of the human nestin protein corresponding to residues 1464-1614.
Purity: Purified by Protein G affinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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