Anti-Naked2 (Nkd2)

Anti-Naked2 (Nkd2)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N0021.100 100 µl - -

4 - 22 business days

496.00€
 
Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of... more
Product information "Anti-Naked2 (Nkd2)"
Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/beta-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/beta-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-alpha to the basolateral plasma membrane of polarized epithelial cells (6). , Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight., IImmunoprecipitation: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: N0021

Properties

Application: IP, WB
Antibody Type: Monoclonal
Clone: 8A19(C36D3)
Host: Rabbit
Reactivity: Mouse
Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding Gly47 of mouse Naked2.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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