Anti-NADPH Oxidase Organizer 1 (NOXO1, MGC20258, P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5)

Anti-NADPH Oxidase Organizer 1 (NOXO1, MGC20258, P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
N0016-30.50 50 µg - -

4 - 22 business days

713.00€
 
NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and... more
Product information "Anti-NADPH Oxidase Organizer 1 (NOXO1, MGC20258, P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5)"
NOXO1 (Nox organizing protein 1) and NOXA1 (Nox Activating protein 1) are homologs of p47phox and p67phox. p47phox functions in phagocytes as an essential organizing protein mediating the binding of other regulatory proteins during activation of the phagocyte oxidase, and its translocation to the membrane is triggered upon cell activation by hyperphosphorylation, which relieves autoinhibition of SH3 and PX domains. NOXO1 lacks an auto inhibitory region and phosphorylation sites that are present in p47phox. Co-transfection of Nox1, NOXO1 and NOXA1 reconstitutes ROS (reactive oxygen species) generation in HEK 293 cells in the absence of cell stimulation. NOXO1 binds to the phosphatidylinositol (PtdIns) lipids PtdIns 3,5-P2, PtdIns 5-P and PtdIns 4-P. Unlike p47phox, which is located in the cytosol of resting cells and translocates to the plasma membrane where gp91phox is located, NOXO1 colocalizes with Nox1 in the membranes of resting cells. This localization of NOXO1 is dictated by its PX domain, since this domain but not the remainder of the molecule localizes to membranes. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 5-10ug/ml, Western Blot: 1:2000-1:8000, ELISA: 1:20,000-1:100,000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Anti-Nox organizer 1, Anti-Nox-organizing protein 1, Anti-NADPH oxidase organizer 1, Anti-NADPH oxidase regulatory protein, Anti-SH3 and PX domain-containing protein 5
Supplier: United States Biological
Supplier-Nr: N0016-30

Properties

Application: ELISA, IF, IHC, WB
Antibody Type: Polyclonal
Host: Rabbit
Reactivity: Human (Expected: Human)
Immunogen: Synthetic peptide, KLH-conjugated. Epitope: Amino acids 238-252 of human NOXO1 protein.
Format: Affinity Purified

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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