Anti-Mucin 16 (MUC16, MUC-16, CA125, CA-125, CA125 Ovarian Cancer Antigen, Cancer Antigen 125, FLJ14

MUC16/CA125 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16/CA125 is a high molecular mass glycoprotein. According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains: A-domain (Oc125), B-domain (M11), C-Domain (Ov197). The CA-125 antigen is present on a mucin-like glycoprotein of >200kD and is detectable on the apical surface of normal endometrium and endocervix. , CA 125, a high-molecular-weight mucin, was first defined in 1981 by the monoclonal antibody OC125. It is a large glycoprotein which is anchored to the epithelium by a transmembrane domain and is released into the extracellular space by enzymatic cleavage. CA 125 is composed of an N-terminal domain, a tandem repeat region, and a short cytoplasmic tail. The N-terminal domain consists of 1637aa, the tandem repeat domain is made up of 40-60 repeats of 156aa which encompass an interactive disulfide bridged cysteine-loop and the site of OC125 and M11 binding and the cytoplasmic tail of CA125 consists of 256aa. It is also highly enriched in serine and threonine residues, consistent with its recent designation as the MUC16 mucin. The gene encoding the glycoprotein CA125 maps to 19p13.2 region of human chromosome. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.