Anti-MBD3, CT (Methyl-CpG-binding Domain 3, Methyl-CpG-binding Protein MBD3)

Anti-MBD3, CT (Methyl-CpG-binding Domain 3, Methyl-CpG-binding Protein MBD3)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
M2475-50C.200 200 µl - -

3 - 19 business days*

728.00€
 
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is... more
Product information "Anti-MBD3, CT (Methyl-CpG-binding Domain 3, Methyl-CpG-binding Protein MBD3)"
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-1:500, Immunohistochemistry: 1:50-1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-Methyl-CpG-binding protein MBD3, Anti-Methyl-CpG-binding domain protein 3
Supplier: United States Biological
Supplier-Nr: M2475-50C

Properties

Application: ELISA, IHC, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Species reactivity: human, mouse
Immunogen: Synthetic peptide corresponding to amino acid residues surrounding E279 of human MBD3 (KLH).
Format: Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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