Anti-Mahogany (Attractin)

The Mahogany (mg) mutations suppress the effect of lethal yellow including obesity. The Mahogany locus does not suppress the obese phenotype of the MC4R null allele or those of monogenic obese models (Lepdb, tub, and Cpefat). However, mahogany can suppress diet-induced obesity. Mahogany (mg) gene, Mgca, encodes a 1428aa single transmembrane protein that is expressed in many tissues including pigment cells and hypothalamus. The extracellular domain of the Mgca protein is the ortholog of human attractin, a circulating molecule produced by activated T-cells implicated in immune cell interaction. The short cytoplasmic tail contains a site that is conserved between C. elegans and mammals. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well. , Western Blot: 1-10ug/ml using ECL., Optimal dilutions to be determined by the researcher. Control Peptide: M2108-03: Mahogany, Mouse (Attractin), Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.