Anti-LOX (LYOX, Lysyl Oxidase, MGC105112, Protein-Lysine 6-Oxidase)

Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extracellular matrix. In addition, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility, and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000. A band is seen at ~58kD. May see bands at ~32kD (mature, secreted form), 50kD and 58kD (pro and glycosylated forms) since L3514-50B is a highly glycosylated protein., Immunohistochemistry (Paraffin): 1:200, Optimal dilutions to be determined by the researcher. Positive Control: Human kidney lysate, mouse kidney lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.