Anti-LOPP (Lysyl Oxidase Pro-peptide)

Lysyl oxidase (LOX) is an extracellular enzyme that is critical for the normal biosynthesis of collagens and elastin. LOX is synthesized as a 50kD precursor, secreted into the extracellular matrix and subsequently proteolytically processed to a 30kD active enzyme and an 18kD propeptide. LOX also reverts ras-mediated transformation and its expression is down-regulated in human cancers. Most recently, the lysyl oxidase pro-peptide (LOPP) has been associated with tumor suppression, as it inhibits ras-dependent cellular transformation. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 0.5-1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: MC3T3-E1 lysate, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. ,