Anti-Jumonji Domain Containing 1B, 2B(JMJD1B, JHDM2B, Jumonji JmjC domain-containing histone demethy

Anti-Jumonji Domain Containing 1B, 2B(JMJD1B, JHDM2B, Jumonji JmjC domain-containing histone demethy
Item number Size Datasheet Manual SDS Delivery time Quantity Price
J7876-46.100 100 µl - -

3 - 19 business days*

514.00€
 
The methylation state of lysine residues in histone proteins is major determinant for formation... more
Product information "Anti-Jumonji Domain Containing 1B, 2B(JMJD1B, JHDM2B, Jumonji JmjC domain-containing histone demethy"
The methylation state of lysine residues in histone proteins is major determinant for formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development (1,2). Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins (3). The JmjC domain of several proteins has been shown to catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and alpha-ketoglutarate (3). Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into seven separate families (3). The JMJD1 (Jumonji domain-containing protein 1) family, also known as JHDM2 (JmjC domain-containing histone demethylation protein 2) family, contains four members: hairless (HR), JMJD1A/JH- DM2A, JMJD1B/JHDM2B, JMJD1C/JHDM2C. Hairless is expressed in the skin and brain and acts as a co-repressor of the thyroid hormone receptor (4-6). Mutations in the hairless gene cause alopecia in both mice and humans (4,5). JMJD1A is expressed in meiotic and post-meiotic male germ cells, contributes to androgen receptor-mediated gene regulation, and is required for spermatogenesis (7-9). It has also been identified as a downstream target of OCT4 and STAT3 and is critical for the regulation of self-renewal in embryonic stem cells (10,11). JMJD1B is a more widely expressed family member and is frequently deleted in myeloid leukemia (12). JMJD1C (also known as TRIP8) is a co-factor of both the androgen receptor and thyroid receptor and has a potential link to autism (13-15). Members of the JMJD1/JHDM2 family have been shown to demethylate mono-methyl and di-methyl histone H3 (Lys9) (3,8). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:100, Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Immunoprecipitation: 1:25, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: J7876-46

Properties

Application: IF, IP, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic peptide (KLH- coupled) corresponding to the human JMJD1B protein.
Purity: Purified by Immunoaffinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow. more
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Anti-Jumonji Domain Containing 1B, 2B(JMJD1B, JHDM2B, Jumonji JmjC domain-containing histone demethy"
Write a review
or to review a product.
Viewed