Anti-Interleukin 1 Receptor Type II (IL-1RII, IL-1R2, CD121b, CDw121B, IL-1R beta, IL1-Rb, MGC47725,

Interleukin-1 receptor II (IL-1 RII), also known as CD121b, is an immunoglobulin superfamily receptor that binds the pleiotropic cytokines, IL-1alpha and IL-1beta. It acts as an IL-1 antagonist because it has a short cytoplasmic domain and does not transduce IL-1 signals. IL-1 RII exists both as a transmembrane protein and as a soluble form that is released by proteolysis. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 cells, Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells from a mouse immunized with purified, insect cell line Sf21-derived, recombinant human IL-1R II (aa14-343). Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. , 2. After blocking, add 10ul of I7663-25H2 to 1x10e6 cells and incubate for 30 minutes at RT., 3. Remove unreacted antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10) (note: whole blood will require an RBC lysis step at this point using any commercially available lysing reagent). , 4. Resuspend cells in Flow Cytometry Staining Buffer (F4568-10) for final flow cytometric analysis., 5. As a control for analysis, cells in a separate tube should be treated with PE-labeled mouse IgG21 antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.