Anti-IL-27 (human), Azide Free Clone B-G44 [Mouse]

Anti-IL-27 (human), Azide Free Clone B-G44 [Mouse]
Item number Size Datasheet Manual SDS Delivery time Quantity Price
DC-853.970.000 200 µg (200µl) -

3 - 8 business days*

204.00€
DC-853.970.005 500 µg (500 µl) -

3 - 8 business days*

432.00€
 
Specificity: Recognises both natural and recombinant human IL-27, Format: Phosphate-buffered... more
Product information "Anti-IL-27 (human), Azide Free Clone B-G44 [Mouse]"
Specificity: Recognises both natural and recombinant human IL-27, Format: Phosphate-buffered saline. Sterile-filtered through 0.22 µm. Carrier and preservative free, Storage: Store at +2-8°C for 12 months. For longer storage, freeze aliquots, Application Notes: Suitable for ELISA Protein function: Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN- gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27- mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP- 10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication. [The UniProt Consortium]
Keywords: Mouse Anti-Human IL-27 Monoclonal Antibody, Azide Free Clone B-G44, Anti-p28, Anti-IL27, Anti-IL27A, Anti-IL27-A, Anti-IL-27-A, Anti-Interleukin-30, Anti-IL-27 subunit alpha, Anti-Interleukin-27 subunit alpha
Supplier: Diaclone
Supplier-Nr: 853.970

Properties

Application: ELISA
Antibody Type: Monoclonal
Clone: B-G44
Conjugate: No
Host: Mouse
Species reactivity: human
Immunogen: Recombinant human IL-27

Handling & Safety

Storage: +4°C
Shipping: +20°C (International: °C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H300, H310, H410, EUH032
P Phrases: P262, P264, P270, P273, P280, P321, P330, P391, P301+P310, P302+P352, P361+P364, P405, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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