Anti-ICOS (Inducible T-cell Costimulator, Inducible T-cell Co-stimulator, Activation-inducible Lymph

The inducible costimulator CD278 (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells (1). Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement (2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS. Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain (4). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.