Anti-Histone H3K9Ac Monoclonal Antibody (RM161)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Acetylation of histone H3 at lysine 9 (H3K9Ac) is associated with active gene transcription and can recruit the super elongation complex to chromatin through direct binding with the AF9 and ENL subunits. It increases at the IFN-beta promoter in HeLa cells upon infection with Sendai virus. Acetylation of histone H3 at lysine 9 decreases following induction of DNA damage in HeLa and U2OS cells. Cayman's Histone H3K9Ac Monoclonal Antibody (RM161) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Acetylated Histone H3 Lysine 9. Immunogen: Peptide corresponding to H3K9Ac. Clone Designation: RM161. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, ICC, IHC, multiplex-based assays, WB. Cross Reactivity: (+) H3K9Ac, (-) H3K4Ac, (-) H3K14Ac, (-) H3K18Ac, (-) H3K23Ac, (-) H3K27Ac, (-) H3K36Ac, (-) H3K56Ac, (-) H3K79Ac, (-) H3K122Ac.