Anti-Hevein

Rubber (cis-1,4-polyisoprene), an isoprenoid polymer, is produced in about 2000 plant species. It is the raw material of choice for gloves, tiresand other industrial products. Allergy to natural rubber latex products has been recognized as a serious medical problem especially among health care workers and children with spina bifida. Interestingly, there appears to be significant clinical and immunochemical cross-reactivity between some latex proteins and allergens in certain fruits and vegetables, such as banana, kiwi, avocado, and potato, and patients with fruit and vegetable allergy may be at increased risk for reacting to latex proteins. Proteins leached from the gloves or extractable latex proteins, ranging in size from 5-200kD, have been shown to be involved in eliciting type I hypersensitivity. Hevea brasiliensis has been the only commercial sourceof natural rubber mainly because of its abundance in the tree,its quality, and the ease of harvesting. Latex is produced in laticifers, which are specialized structures that consist of anastamosed latex-producing cells. Harvested Hevea latex is a complete cytosol with high protein content. Several potential allergens have been identified in Hevea latex, including rubber elongation factor (REF or Hev b 1), hevamine-1,3-glucanase (Hev b 2), hevein preprotein (HEV1), a 24-kD rubber particle-associated protein (RPP) (Hev b 3), and a component of the microhelix protein complex (Hev b 4), a 16kD acidic protein Hev b 5, Hev b 6, and Hev b 7. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1-10ug/ml using ECL technique., ELISA: 0.5-1ug/ml, H2034-80B Control peptide can be used to coat ELISA plates at 1ug/ml., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.