Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, GAPD, HGNC:4141, G3PD, MGC88685)

Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, GAPD, HGNC:4141, G3PD, MGC88685)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
G8140-16P.100 100 µl - -

3 - 19 business days*

498.00€
 
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved... more
Product information "Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, GAPD, HGNC:4141, G3PD, MGC88685)"
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:20,000, Optimal dilutions to be determined by the researcher. Positive Control: Mouse liver, HeLa, Jurkat, NIH3T3, K562 and HepG2 cell lysates. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: G8140-16P

Properties

Application: ELISA, WB
Antibody Type: Monoclonal
Clone: 8L49
Conjugate: No
Host: Mouse
Reactivity: Human, Mouse
Immunogen: Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3).
Purity: Purified by immunoaffinity chromatography.
Format: Affinity Purified

Handling & Safety

Storage: +4°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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