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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
G8140-16P.100 | 100 µl | - | - | 3 - 19 business days* | 498.00€ |
You can also order using info@biomol.com or FAX at 0049-40-85326022
Bigger quantity required? Request Bulk
Bigger quantity required? Request Bulk
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved... more
Product information "Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, GAPD, HGNC:4141, G3PD, MGC88685)"
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggest that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. During the last decade a lot of findings appeared concerning the role of GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age- related neuronal diseases, such as Alzheimer's and Huntington's disease. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:20,000, Optimal dilutions to be determined by the researcher. Positive Control: Mouse liver, HeLa, Jurkat, NIH3T3, K562 and HepG2 cell lysates. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: | United States Biological |
Supplier-Nr: | G8140-16P |
Properties
Application: | ELISA, WB |
Antibody Type: | Monoclonal |
Clone: | 8L49 |
Conjugate: | No |
Host: | Mouse |
Reactivity: | Human, Mouse |
Immunogen: | Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3). |
Purity: | Purified by immunoaffinity chromatography. |
Format: | Affinity Purified |
Database Information
KEGG ID : | K00134 | Find alternatives |
UniProt ID : | P04406 | Find alternatives |
Gene ID | GeneID 2597 | Find alternatives |
Handling & Safety
Storage: | +4°C (-20°C Glycerol) |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
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