Anti-FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF-3-alpha, HNF3A, HNF-3A, MGC33105

FoxA1 is a 473aa, 49kD (predicted) protein of the forkhead nuclear transcription factor family. It regulates transcription downstream of the estrogen receptor and other steroid hormone receptors. FoxA1 is expressed in breast luminal cells and correlates with proliferation in breast cancer cell lines. Within the sequence used as an immunogen, human FoxA1 shares 93-86aa identity with mouse and rat FoxA1, respectively. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunocytochemistry: 8-25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.