Anti-FLASH (FLICE-associated Huge Protein, CASP8AP2, CED-4, FLJ11208, KIAA1315, RIP25)

Fas is a cell-surface receptor molecule that relays apoptotic signals into cells. When Fas is activated by the binding of its ligand, the proteolytic protein caspase-8 is recruited to a signaling complex known as DISC by binding to a Fas-associated adapter protein. A new protein, FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of the DISC signaling complex and is necessary for the activation of caspase-8 in Fas-mediated apoptosis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.