Anti-Feline Immunodeficiency Virus, p24 gag (FIV)

The lentivirus FIV, responsible for a progressive and debilitating immune deficiency syndrome in domestic cats, similar to that caused by the human Immunodeficiency Virus (HIV), is a complex retrovirus with a tightly-packed genome, containing the structural genes gag, env and pro-pol and the accessory genes vif, rev and ORF-A/2. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 10ul labels 1x10e6 in 100ul. Requires membrane permeabilisation, Immunohistochemistry: Frozen sections. Sensitive to formaldehyde fixation and tissue processing. Use acetone fixation for frozen sections. Not suitable for use on paraffin sections., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.