Anti-Cre Recombinase

Cre-recombinase is a 38kD DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family. C7920-01 is an important tool for the identification of Cre recombinase in different protein expression systems. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.05-0.2ug/ml, Western Blot: 0.1-1.0ug/ml , Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.