Anti-COTL1 (CLP, Coactosin-like Protein)

COTL1 (Coactosin-like Protein) is both a cytoplasmic and plasma­appearing 15-16kD member of the coactosin subfamily, ADF/Actin Depolymerizing Factor family of actin-binding proteins. It is widely expressed, and found in cell types such as neutrophils, and tissues such as placenta, lung and kidney. Functionally, COTL1 interacts noncovalently with both F-actin and 5­lipoxygenase/5LO. These interactions appear to be mutually exclusive. A COTL1:F­actin interaction leads to actin binding without actin polymerization, while a 5LO:COTL1 interaction has two potential outcomes, first, 5LO sequesters COTL1, leading to a failure of actin binding, and second, COTL1 can serve as a scaffold for 5LO activity, facilitating the production of either 5HPETE or LTA4. Human COTL1 is 142 amino acids (aa) in length. It is principally composed of one ADF-H domain (aa2-130) that possesses a utilized phosphorylation site at Ser115, and two acetylation sites at Lys102 and Lys126. COTL1 may form noncovalent homodimers and oligomers, but not when complexed to F­actin. There is one potential isoform variant that shows a 106aa substitution for aa1-53. Full­length human and mouse COTL1 share 95% aa sequence identity. Applications: Suitable for use in ELISA, Western Blot, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunocytochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.