Anti-Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alph

Anti-Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alph
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C7510-53N.100 100 µg - -

3 - 19 business days*

854.00€
 
COL4A1 (collagen IV a1) is a 185kD member of the type IV collagen family. It is a secreted... more
Product information "Anti-Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alph"
COL4A1 (collagen IV a1) is a 185kD member of the type IV collagen family. It is a secreted glycoprotein that is expressed by multiple cell types, including fibroblasts, keratinocytes and endothelial cells. Two COL4A1 molecules interact with a 170kD a2 chain to form a collagen IV triple helix. This helix further interacts with other helices to generate covalent oligomers that form a scaffold in the basement membrane. Mature human COL4A1 is 1642aa in length. It has an N-terminal "7S" proregion aa28-172, a central collagenous domain that contains multiple Gly-based repeats aa173-1440, and a C-terminal domain that is proteolytically cleaved to generate a 25-28kD NC1 globular segment that has potent antiangiogenic activity aa1441-1669. Multiple splice forms exist. One shows a deletion of aa499-849, a second shows a seven aa substitution for aa513-1669, and a third shows a seven aa substitution for aa958-1669. Over aa1441-1669, human COL4A1 is 97% aa identical to mouse COL4A1. Applications: Suitable for use in Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 8-25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Anti-Collagen alpha-1(IV) chain
Supplier: United States Biological
Supplier-Nr: C7510-53N

Properties

Application: ICC
Antibody Type: Monoclonal
Clone: 11n50
Conjugate: No
Host: Mouse
Species reactivity: human
Immunogen: Recombinant corresponding to aa1441-1669 from human Collagen IV a1 expressed in NSO cells (02462).
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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