Anti-CLPB (ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of pot

Anti-CLPB (ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of pot
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C2279-10.100 100 µg - -

3 - 19 business days*

414.00€
 
Applications: |Suitable for use in ELISA. Other applications not tested.||Recommended... more
Product information "Anti-CLPB (ClpB caseinolytic peptidase B homolog (E. coli), FLJ13152, HSP78, SKD3, suppressor of pot"
Applications: , Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:32,000. , Western Blot: 0.1ug/ml. Bands detected at ~50kD and 48kD in lysates of HeLa and HepG2. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See C2279-10-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Keywords: Anti-Caseinolytic peptidase B protein homolog, Anti-Suppressor of potassium transport defect 3
Supplier: United States Biological
Supplier-Nr: C2279-10

Properties

Application: ELISA
Antibody Type: Polyclonal
Conjugate: No
Host: Goat
Reactivity: Human
Immunogen: A synthetic peptide from the C-terminus of aa-DKDSKTRRLDIRAP
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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