Anti-Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit A, CAF-1 Sub

Anti-Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit A, CAF-1 Sub
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C5069-94C.100 100 µl - -

3 - 19 business days*

727.00€
 
Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de... more
Product information "Anti-Chromatin Assembly Factor 1 (CAF1, CAF-1, CAF, Chromatin Assembly Factor 1 Subunit A, CAF-1 Sub"
Chromatin assembly factor 1 (CAF-1) is a histone H3/H4 chaperone complex that functions in de novo assembly of nucleosomes during DNA replication and nucleotide excision repair (1). Nucleosome assembly is a two-step process, involving initial deposition of a histone H3/H4 tetramer onto DNA, followed by the deposition of a pair of histone H2A/H2B dimers (1). CAF-1 interacts with PCNA and localizes to DNA replication and DNA repair foci, where it functions to assemble newly synthesized histone H3/H4 tetramers onto replicating DNA (2-6). Assembly of histone H2A/H2B dimers requires additional assembly factors. The CAF-1 complex consists of three proteins: CHAF1A (p150), CHAF1B (p60) and RBAP48 (p48 or RBBP4). CHAF1A and CHAF1B proteins are specific for the CAF-1 complex, while RBAP48 is a component of multiple chromatin modifying complexes (1). CHAF1A and CHAF1B expression levels correlate with cellular proliferation and both proteins are significantly down-regulated in quiescent cells (7). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:200, Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: Anti-CAF-1 subunit A, Anti-CAF-I 150 kDa subunit, Anti-Chromatin assembly factor 1 subunit A, Anti-Chromatin assembly factor I p150 subunit
Supplier: United States Biological
Supplier-Nr: C5069-94C

Properties

Application: IF, IP, WB
Antibody Type: Monoclonal
Clone: 11C1020(D77D5)
Conjugate: No
Host: Rabbit
Species reactivity: human, monkey
Immunogen: Synthetic peptide corresponding to residues surrounding Thr293 of human CHAF1A.
Format: Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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