Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
C4164-50.100 | 100 µg | - | - |
3 - 19 business days* |
916.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Human CHIT-1 (chitotriosidase-1, also chitinase-1) is a 50kD, secreted glycoprotein that belongs... more
Product information "Anti-Chitotriosidase (Chitinase 1, CHIT-1)"
Human CHIT-1 (chitotriosidase-1, also chitinase-1) is a 50kD, secreted glycoprotein that belongs to glycosylhydrolase family #18. It is synthesized by macrophages, and may serve to degrade chitin (poly-N-acetylglucosamine)-containing pathogens such as fungi and nematodes. Mature CHIT-1 is 435 amino acids (aa) in length, and contains a catalytic domain (aa22-372) followed by a chitin-binding region (aa373-466). Very high levels of CHIT1 are found in the plasma of patients with Gaucher's disease type I. Mature human CHIT-1 is 79% aa identical to mouse CHIT-1. Applications: Suitable for use in Direct ELISA, Immunoprecipitation and Neutralization. Other applications not tested. Recommended Dilutions: ELISA (Direct): 0.5-1.0ug/ml. Detection limit for rhCHIT1 is approximately 2ng/well., Immunoprecipitation: 25ug/ml to immunoprecipitate rhCHIT1 from conditioned cell culture medium. , Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 min. The enzyme was then assayed with 4-MU-beta-D-N,N',N''-triacetylchitotriose. 50% of the enzymatic activity was inhibited by the antibody at approximately 56ng/ml (IC 50) under conditions in which the enzyme was present at 20ng/ml and the substrate concentration was 0.01mM. IC 50 was achieved at approximately 1:1 molar ratio of the antibody to the enzyme., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: | Anti-CHIT1, EC=3.2.1.14, Anti-Chitinase-1, Anti-Chitotriosidase-1 |
Supplier: | United States Biological |
Supplier-Nr: | C4164-50 |
Properties
Application: | ELISA, IP, Neutr. |
Antibody Type: | Monoclonal |
Clone: | 7H206 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | NS0-derived rhCHIT-1 |
Format: | Affinity Purified |
Database Information
KEGG ID : | K01183 | Matching products |
UniProt ID : | Q13231 | Matching products |
Gene ID | GeneID 1118 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
Viewed