Anti-CD95 (Fas)

Anti-CD95 (Fas)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C2442-21E.20 20 µl - -

3 - 19 business days*

335.00€
C2442-21E.100 100 µl - -

3 - 19 business days*

574.00€
 
Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an... more
Product information "Anti-CD95 (Fas)"
Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenophathy and splenomegaly as a result of decreased apoptosis in CD4-CD8-T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type 2 membrane protein of 280aa (runs at ~40kD upon glycosylation) that belongs to the TNF family which also includes TNF-alpha, TRAIL and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to the execution of apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells and at immunologically privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26kD trimeric soluble form (8,9). , , Applications: , Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. , Immunohistochemistry (paraffin): 1:480, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: C2442-21E

Properties

Application: IHC, WB
Antibody Type: Polyclonal
Conjugate: No
Host: Rabbit
Reactivity: Human
Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues surrounding Lys259 of human Fas, within the intracellular region.
Purity: Purified
Format: Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Anti-CD95 (Fas)"
Write a review
or to review a product.
Viewed