Anti-CD46 (Membrane cofactor protein, MCP) (FITC)

CD46, also designated membrane cofactor protein, is a 45-70kD protein with genetic and tissue-specific heterogeneity. It is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to down-regulate the activation of complement on host tissue. It performs this function by serving as a cofactor which binds to C3b and C4b. This binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. It also serves as a receptor for measles virus. Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. The isoforms differ in the length. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR). Applications: Suitable for use in FLISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Functional studies. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 stains the extracellular domain of CD46., Western Blot: 1:50 using non-reduced sample treatment and SDS-PAGE detects a band of 45-70kD (ref1,2), Immunohistochemistry: 1:10, Immunoprecipitation: 1:10, 10e7 cells were lysed and immunoprecipitated with 25ug C2401-02D and 25ug protein G sepharose., Optimal dilutions to be determined by the researcher. Recommended Negative Control: Erthyrocytes, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.