Anti-CD41 (integrin alpha IIb chain, gpIIb-IIIa, Platelet Membrane gpIIb-IIIa Complex)

CD41 is the integrin alpha IIb chain, also called gpIIb, which is non-covalently associated with the integrin beta 3 chain, also called gpIIIa (CD61), forming the gpIIbIIIa complex. Antibody reacts speci?cally with normal platelets and megakaryocytes but studies have shown that it does not react with Glanzmann's thrombasthenic platelets which lack the membrane glycoprotein gpllbllla. Does not inhibit the platelet aggregation and secretion induced by collagen, arachidonic acid and thrombin. Does not inhibit the ?brinogen binding to human platelets induced by ADP, arachidonic acid and PAF. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 10-20ug/ml will allow visualization of NP 40 solublized protein on human normal platelets. Under reducing conditions they will bind against the human platelet membrane glycoproteins IIb/IIIa complex, which have a molecular weights of ~130,000 (IIb) and 115,000 (IIIa). , Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.