Anti-CD326 (Low Endotoxin)

Epithelial cell adhesion molecule (Ep-CAM), a 34kD cell surface antigen otherwise known as CD326, MOC31 and Ber-EP4. CD326 is a type 1 transmembrane glycoprotein. It is expressed on the basolateral cell membrane of the majority of epithelial tissues, with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells. Applications: Suitable for use in Flow cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot..Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:10-1:200 Use 10ul of the suggested working dilution to label 1x10e6 cells in 100ul. , Immunohistochemistry: paraffin and frozen tissues. Requires protein digestion pre-treatment of paraffin sections e.g. trypsin. , Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized mice were fused with cells of the mouse SP2/0 myeloma cell line. Positive Control: Large Bowel Tissue , Recommended Secondary Antibodies:, I1904-65W: IgG, F(ab')2 (HRP) Pab RbxMo, I1904-66C: IgG, F(ab')2, X-Adsorbed (HRP) Pab RbxMo, I1904-66A: IgG, F(ab')2 (FITC) Pab Rb xMo, Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.