Anti-CD22 (CD22 Antigen, B Cell Receptor CD22 Precursor, B Lymphocyte Cell Adhesion Molecule, BL-CAM

In Western Blot (e.g. Raji cell line) stained in the non-reduced sample only the upper band (cca 140kD) but not the weaker 130kD zone which was stained by several CD22 standards (these stained both zones). It was therefore assumed that the 140kD zone corresponds to the so called CD22 beta (long)isoform. This assumption was not rigorously tested on transfectants expressing various alternative spliced forms of CD22. Cross-blocked only one of the tested CD22 standards (OTH228, which was described as recognizing uniquely epitope "E" in the 4th workshop), it did not cross-block mAbs RFB-4, CLB22/1 and CLB-Bly1. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.