Anti-CD14 (CD14 Antigen, CD14 Molecule, Monocyte Differentiation Antigen 14, Myeloid Cell Specific L

CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Anti-CD14 antibody labels Kupffer cells in liver sinusoids. In lymphoid tissues, dendritic cells are distinctly stained. Most other normal tissues are negative. This antibody labels monocyte-macrophages and Langerhans cells in Langerhans cell histiocytosis. Tumor cells are positive in monocytic leukemia and true histiocytic lymphomas for CD14. Sinusoidal histiocytes express CD14 and CD 169, whereas most of the other monocyte-derived cells in reactive lymph node lack these markers. Anti-CD14 labels numerous diffuse large B-cell lymphomas and splenic marginal zone lymphoma but not in other B- cell lymphomas. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin, frozen): 1:50-1:200, Optimal dilutions to be determined by the researcher. Positive Control: Tonsil, diffuse large B-cell lymphoma (DLBCL), Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.