Anti-Carbohydrate Sulfotransferase 4 (CHST4, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase

Anti-Carbohydrate Sulfotransferase 4 (CHST4, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C1103-04C.100 100 µg - -

3 - 19 business days*

678.00€
 
The CHST family is comprised of 14 enzymes in human. All members of this family are... more
Product information "Anti-Carbohydrate Sulfotransferase 4 (CHST4, Carbohydrate (N-acetylglucosamine 6-O) sulfotransferase"
The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins (1). Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6 O or 4O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (2). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (3). CHST4, also, known as high endothelial cells Nacetylglucosamine 6Osulfotransferase (HECGlcNAc6ST), or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L selectin, ligands (4). It has a catalytic preference for core 2-branched mucin-type O glycans, but also has activity toward core 3 type of O glycan 5). Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog. Applications: Suitable for use in Neutralization, ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml, Western Blot: 1.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Anti-Gst3, EC=2.8.2.-, Anti-Gn6st-2, Anti-Carbohydrate sulfotransferase 4, Anti-N-acetylglucosamine 6-O-sulfotransferase 2, Anti-High endothelial cells N-acetylglucosamine 6-O-sulfotransferase
Supplier: United States Biological
Supplier-Nr: C1103-04C

Properties

Application: ELISA, IP, FA (Neutr.), WB
Antibody Type: Polyclonal
Conjugate: No
Host: Sheep
Reactivity: Human
Immunogen: Human CHST4.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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