Anti-Calmodulin-dependent Protein Kinase Type 1 (CaM-KI, CaM kinase I alpha, CAMKI-alpha)

Anti-Calmodulin-dependent Protein Kinase Type 1 (CaM-KI, CaM kinase I alpha, CAMKI-alpha)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
C1035-03L.100 100 µg - -

3 - 19 business days*

557.00€
 
CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional... more
Product information "Anti-Calmodulin-dependent Protein Kinase Type 1 (CaM-KI, CaM kinase I alpha, CAMKI-alpha)"
CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional regulation, hormone production, translational regulation, regulation of actin filament organisation and neurite outgrowth. CaM kinase I is involved in calcium-dependent activation of the ERK pathway. It phosphorylates proteins with the substrate consensus sequence [MVLIF}-x-R-x(2)-[ST]-x(3)-[MVLIF]. It is activated by calcium ions or calmodulin. It reaches its maximum catalytic activity when phosphorylated by CAMKK1 or CAMKK2. CaM kinase I has a length of 370 amino acids (unprocessed precursor) and a predicted molecular weight of 41.3KDa. It is a monomer that exists in many tissues and is located within the cytoplasm or nucleus. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the CaMK subfamily. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: Denaturing conditions in HL-60 lysates, ELISA (Sandwich): Detection limit 0.3ng/ml with recombinant GST tagged CaM Kinase 1, Optimal dilution determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Keywords: Anti-CAMK1, EC=2.7.11.17, Anti-CaMKI-alpha, Anti-Calcium/calmodulin-dependent protein kinase type 1
Supplier: United States Biological
Supplier-Nr: C1035-03L

Properties

Application: ELISA, WB
Antibody Type: Monoclonal
Clone: 9G328
Conjugate: No
Host: Mouse
Reactivity: Human
Immunogen: Partial recombinant protein of human CaM kinase 1 (aa 271-370) with a GST tag.
Format: Affinity Purified

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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