Product information "Anti-Cadherin P (Cadherin-3, placental, CDH3), clone 3G51"
P-cadherin is a 120kD Ca2+-dependent cell adhesion molecule, which appears to be expressed in cells having high growth activity and/or high differentiation potential. In the early mouse embryo, P-cadherin has been detected in the extra-embryonic ectoderm, visceral endoderm, Iateral plate mesoderm, and notocord. In the late embryo, P-cadherin is detected in the placenta, epidermis and other epithelial tissues, the pigmented retina, and the mesothelium. P-cadherin displays a rather unique distribution pattern in human tissues in that its expression is not detected in placental tissue. In humans, expression is restricted to epithelial tissues where it appears to be restricted to the basal or lower layers of strati?ed epithelia. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Also suitable as an inhibitor of P-cadherin dependent cell-cell contact. Other applications not tested. Recommended Dilutions: Immuno?uorescence: 2-5ug/ml , ELISA: 0.5ug/ml. Using immobilized A431 cells. , Western Blot (Native): 0.5-2ug/ml , Immunohistochemistry (FFPE): 2ug/ml, Requires heat induced epitope retrieval with citrate buffer, pH 6.0, prior to staining., Inhibition of P-cadherin-dependent cell-cell contact: 100ug/ml , Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cell line P3-X63-Ag8-U1, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.