Anti-Cadherin E (Cadherin-1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Cadherin E is a 123kD molecule of the cadherin family. One of the epithelial cell adhesion molecules, Cadherin E, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso-or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, Cadherin E plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Cadnerins constitute a familly of transmembrane glycoproteins involved in Ca2+ dependent cell-cell interactions. The members of this family are diferentially expressed in various tissues. One of the best characterized cadherins in Cadherin E, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40kD transmembrane and cytoplasmic part. Cadherin E is a functional antibody which inhibits cell-cell adhesion. Antibodies against Cadherin E are suitable to characterize epithelial cells. Cadherin E is involved in adhesion of epithelial cells (mostly homotypic and homophilic) and is expressed on immature erythroid cells. In addition, it blocks the interaction of Cadherin E molecules and therefore is suitable to study Cadherin E-mediated signal transduction. Applications: Suitable for use in Flow Cytometry, Western Blot, Functional blocking of Cadherin E. Not recommended for use in Immunoprecipitation. Other applications not tested. Positive Control: MCF-7 membranes, Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.