Anti-CA125 Cancer Antigen (Ovarian Cancer), clone 0.N.37

MUC16/CA125 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16/CA125 is a high molecular mass glycoprotein. According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains: A-domain (Oc125), B-domain (M11), C-Domain (Ov197). The CA-125 antigen is present on a mucin-like glycoprotein of >200kD and is detectable on the apical surface of normal endometrium and endocervix. , Applications: , Suitable for use in Immunohistochemistry (frozen & formalin/paraffin). Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:25-1:50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.